ABSTRACT
Objection To investigate the main influencing factors for extracting total polysaccharides and two active adjuvant components from Poria cocos. Methods Extracting temperature was designed between 20℃-100℃,ratio of material/water was 1/5-1/20 (W/V),extracting time was 1-6 h,extracting frequency was 1-3 times,material size was crude slices~50 mesh,and extracting mode was standing or stirring. The sugar content was determined by phenol-sulfuric acid method and the reletive molecular mass of polysac-charides was determined with gel filtration chromatography. Monosaccharide compositions were analyzed with PMP-derivation and cap-illary electrophoresis. Results ①The extracting temperature should be below 70℃;②The ratio of material/water was suitable be-tween 1/5-1/20;③The extracting time might be within 2 h;④The extracting frequency should be no more than two times;⑤The raw material should be crushed before extracting;⑥The stirring was favorable for extracting polysaccharides. Conclusion The re-sults indicate that the extracting temperature,extracting frequency and stirring mode are important for extracting polysaccharides,and the cushing of the raw materials is helpful to extract the active adjuvant component of polysaccharides from P. cocos in higher yield.
ABSTRACT
Objective To identify the genetic variation and possible sources of Thelazia callipaeda isolates collected from pa-tients in Zunyi City,Guizhou Province. Methods Seven cases of T. callipaeda infection in Zunyi City,2016 were verified, and DNA(s)were extracted from the T. callipaeda's body collected from the thelaziasis patients. A mitochondrial COX1 frag-ment was amplified and sequenced. The sequence alignment and phylogenetical analysis were performed to compare the genetic variation of the gene sequence with the homologous sequences downloaded from Genebank. Results COX1 genes of T. callipae-da were differed among the samples from the seven cases,which had low variation. Conclusion Zunyi City is a new area with endemic of thelaziasis. The isolates from Zunyi City include either Asian origin or European origin of T. callipaeda. Moreover,at least four haplotypes are identified among the seven isolates.